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A 3% HNO3 blank was processed in each batch of samples to control for possible contamination.
A blank was processed in between each GC/MS sample to minimize cross-sample contamination.
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To produce a blank control sample, PP without nucleating agent was processed in the same way.
Calibrators, reagent blanks, and quality-control materials were processed in the same manner as the samples.
Blanks (wells containing medium alone) were processed in parallel, and their values were subtracted from those of the sample plates.
Tubes containing standard-containing acid but no sample were processed in each batch to provide "digestion" blanks.
Blanks were processed with each batch of samples.
The samples were processed in batches of 14 samples, one internal quality control sample, and a blank.
Furthermore, a blank sample was processed to define the baseline.
One hundred microliters of blank plasma from each lot was processed as per the procedure mentioned in sample preparation.
Thereafter, those three spiked samples as well as the fourth blank sample were processed according to the procedure described in " Sample preparation".
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