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Exact(5)
At the same time, the blank was prepared using 1 ml of control sample.
The blank was prepared using the deionised water instead of the L-DOPA solution for each mixture of PPO and trypsin.
For each RT, a blank was prepared using all the reagents except the RNA sample (replaced with an equivalent volume of diethylpyrocarbonate (DEPC -treated water) anDEPC -treateds non-template control in real-time PCR experiments.
The blank was prepared using 1 mL of distilled water.
A blank was prepared using the above-mentioned protocol with plasma replaced by physiological solution.
Similar(55)
Appropriate blanks were prepared using the solvent only in addition to the same amount of DPPH reagent to get rid of any inherent solvent effect.
Appropriate blanks were prepared using the solvent only in addition to the same amount of DPPH reagent to get rid of any inherent solvent activity.
The reaction mixture was incubated at 37°C for 4 min, and then the absorbance was read at 593 nm against a blank that was prepared using distilled water.
After that, the absorbance was determined at 593 nm against a blank that was prepared using distilled water and incubated for an hour instead of 4 min.
Briefly, 200 μL of WSREt, WSFEt or WSLEt (1 mg/ml) was mixed with 1.5 ml of the FRAP reagent, and the reaction mixture was incubated at 37°C for 4 min. The absorbance was then read at 593 nm against a blank that was prepared using distilled water.
Blank was prepared with anthrone reagent and distilled water and the calibration curve was prepared using cellulose in the range of 40 200 µg.
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