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A calibrated curve was constructed by adding a known amount of cefquinome to blank tissue-cage fluid over concentrations ranged from 0.001 μg/mL to 1 μg/mL.
Blank tissues were coming from fish and shrimp bought in Belgian supermarkets.
The representative chromatograms acquired from blank lung tissue, blank lung tissue spiked with the analyte (at LLOQ level) and IS, and lung tissue sample at 1 h after oral administration of Vam3 are shown in Fig. 4. The retention time of Vam3 and IS were 2.80 min and 3.10 min, respectively, in both plasma and tissue samples.
In Fig. 4, LC MS/MS chromatograms are shown for a processed blank muscle tissue sample spiked with penicillins at MRL. Accuracy in muscle tissue varied from 94% to 113% with an intra-day precision (relative standard deviation (RSD r, n = 18) between 5.2% and 13.4% and an inter-day precision (RSDRL, n = 18) between 5.5% and 23%.
Analytical standards were prepared by spiking known amounts of standards into blank lung tissue according to the above extraction procedure.
By analysing 20 different blank porcine tissues, the method was proven to be specific and selective.
There was no peak at the retention time of AICD and IS in the blank plasma and tissue homogenates.
Standards, blank (PBS) and tissue samples (100 μL/well) were incubated for 2 h at room temperature (RT) with agitation (650 rpm).
To avoid false positive results, controls consisting of non-tumour tissue blank paraffin sections and other negative control samples were simultaneously tested in each of the different steps of analysis.
Then the man appeared from behind me with a blank box of tissues.
Sample blanks containing no tissue were generated in the same way, as were occasional tissue samples that were spiked with gallium (used to estimate overall recovery from the procedure).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com