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MDA was calculated using OD as MDA level (nmol/mg protein) = (OD sample − OD blank)/(OD standard solution – OD blank standard solution) × standard solution concentration/sample protein concentration.
LDH cytotoxicity was calculated using OD as LDH cytotoxicity (U/g protein) = (OD sample - OD blank)/(OD standard solution – OD blank standard solution) × standard solution concentration/sample protein concentration.
The concentration detection limit, defined as 3 times the blank standard deviation (3σ), was 0.9 ng mL− 1 for Te.
The concentration detection limits, defined as 3 times the blank standard deviation (3σ), were 0.05 and 0.40 ng mL− 1 for Cd and Pb, respectively.
For those concerned about staying on the right side of the law, the Real Estate Board of New York provides blank standard lease forms, sublease forms and alteration agreements free on its Web site.
(a) System Artifacts: As the first two injections of the day, analyze the blank standard twice in succession.
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Blank, standards and triplicate samples were run during the analysis of samples to check the accuracy and reliability of analysis methods.
Duplicates of each sample were run in two dilutions (50× and 200×) on the plate containing wells for a blank, standards and interassay variance.
Ultrapure water (Millipore, Milli-Q, 16 MΩ cm) was used in the preparation of blanks, standard solutions, and sample dilutions.
Instrumental blanks (standard solution with IS only), showed no traces of interfering compounds.
The concentration of LPO (μmol/L) was calculated according to the following formula: (1) L P O μ m o l / L = m e a s u r e d O D − blanked OD standard OD − blanked OD × standard sample 10 μ m o l / L. Superoxide dismutase (SOD) activity was determined using an SOD Water-Soluble Tetrazolium Salt (WST-1) Assay Kit (Nanjing Jiancheng Bio, Jiangsu, China).
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