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Differences from blank medium and particle controls were observed for all biological responses measured.
Blank medium was used as a negative control.
None of them differred from the blank (medium only).
This value represents just 68% of the cell density obtained using the blank medium.
For half-life studies, cells were treated with cycloheximide (40 μg/ml) at different time points in blank medium.
Complete glucose consumption was only achieved after 48 h fermentation instead of 18 h, as it was in the case of the control blank medium.
Similar(47)
Wells in the last two columns served as blanks (medium only), and 100% survival controls (cells and medium only).
The reason was that Saos-2 and TE-85 did not produce significant amounts of this protein, and there was a minimum difference when compared against blanks (medium from wells containing no cells).
The cells treated by serum-contained blank culture medium served as control.
No interfering peaks were observed in the chromatogram of blank HBSS medium samples, indicating good specificity of the detection method.
Recovery was assessed by spiking blank YES medium with aflatoxin standard solution at a concentration of 10 ng/mL, and it was 92% for aflatoxin B1.
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