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A blank flow cell was used as a negative control surface on flow cell 1.
A blank flow cell was used as a control surface.
Blank flow cells were used to subtract the buffer effect on sensorgrams.
The binding responses were corrected for buffer effects by subtracting responses from a blank flow cell.
For the kinetic experiments, 6 dilutions of chemokines or Evasin-1 WT and F14A were were prepared in running buffer, filtered through a 0.22 µm filter, and injected over the experimental and blank flow cells.
All association and dissociation curves were corrected for non-specific binding by subtraction of control curves obtained from injection of the different analyte concentrations through the blank flow channel.
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The whole staining procedure was applied to blanks for flow cytometry analyses as per recommendation of reference viral staining protocols32 to identify the correct viral gates for analyses and sorting.
Blank on Blank being a non-profit, money it makes this way will ultimately flow back to the journalists who provide the audio.
All kinetic/affinity experiments were carried out at 25°C with at least 5 different analyte concentrations as well as solvent alone (which served as a blank) with a flow rate of 30 μl/min.
Flow cell 1 was left blank as a reference, while flow cells 2 4 were immobilized with DNA by manual injection of DNA stock solutions (flow rate of 1 μL/min) until the desired amount of DNA response units (RU) was obtained (320 330 RU).
The production of seven-sided coins (20- and 50-pence denominations) from circular blanks indicates the extent of the flow of the blank metal within the collar; the striking presses are capable of 600 strokes per minute.
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CEO of Professional Science Editing for Scientists @ prosciediting.com