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Blank determinations were carried out wherever necessary and the corrections were made if required.
For the spectrophotometric analysis, reagent blank determinations were used to correct the instrument readings.
A series of blank determinations were carried out in which the volume of the RN stock solution was replaced with glacial acetic acid.
Based on guideline by Commission Regulation (EC) No. 333/2007 of 28 March 2007, instrument LoD was calculated as the three times the standard deviation of the mean of 21 blank determinations, while instrument LoQ was measured as six times of the standard deviation of the mean of 21 blank determinations (European Commission 2007).
Substrate and α-amylase blank determinations were undertaken under the same conditions.
Two hundred and fifty μL of reference standards and plasma samples were added in duplicate to two square polypropylene 2-mL 96 well plates (Corning Inc ., for the 37°C and blank determinations, respectively.
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One ad, prominently displayed on some New York City subway cars, features a woman staring at the camera with a look of blank determination.
For blank determination, the enzyme solution was replaced by maleate buffer 0.1 M, pH 6.9 (50 µL), and the same procedure as described above was carried out.
LOD and LOQ were calculated as 3.3 σ/s and 10 σ/s, respectively, as per ICH definitions, where, σ is the mean standard deviation of replicate determination values under the same conditions as the sample analysis in the absence of the analyte (blank determination), and s is the sensitivity, namely the slope of the calibration graphs.
For blank determination: 4 ml chilled CO2 saturated water was added to 6.0 ml chilled 0.02 M Tris HCl buffer, pH 8.0 and the time (in seconds) required for the pH to drop from 8.3 to 6.3 was recorded as T0. 1 µg of CAIX-ECD-Fc in 100 µl of PBS was added to 6.0 ml chilled 0.02 M Tris HCl buffer, pH 8.0 immediately before adding of CO2 saturated water.
Lou's darkness, his blank, bleak determination could come from any horrible icon from Norman Bates to Keyser Söze, but Gyllenhaal's performance is so dedicated, and Gilroy's world so determinedly realised that it forces its way to originality.
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