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This partial sequence (assuming blank cycles to be Cys residues) was used to design a degenerate primer pool that was employed successfully in RACE-PCR to clone homologous precursor-encoding cDNA that encoded a mature Kazal protein of 52 amino acid residues with a computed molecular mass of 5892.82 Da.
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However, as previously reported, N-terminal sequences of wbPAG failed to give any signal on cycle 4, this blank cycle being followed by the consensus sequence L-T that is a characteristic of the N-glycosylation site.
The code allows for the prediction of the geometry of the sheared profile, the mechanical state of the sheared zone, the burr height, the force penetration curve, and the wear evolution of the punch versus the number of the blanking cycles.
The findings in this paper may also be easily extended to time-domain resource coordination among macrocells and femtocells as specified in 3GPP Release-10 [22], where the duty cycle of blank subframes [23] may be optimized while jointly considering capacity maximization, fairness, and QoS constraints.
In the case of eICIC, the duty cycle of almost blank subframes has a significant effect on the fairness, but with reduced power subframes and optimized scheduling thresholds, duty cycle has a limited effect on fairness.
Brute-force search method is also used, for example, in [16] to find the optimum REB and duty cycle of almost blank subframes that maximize the rate coverage in HetNets. Figure 9 shows the plots of ρ∗ and ρ′∗ as the functions of α and τ.
Specific binding was assessed by subtracting a blank buffer injection for each cycle.
The results found that following 48 h of exposure, the cell cycle distribution of blank group and vehicle groups exhibited no significant difference, demonstrating that DMSO at very low concentrations had no obvious effect.
The duration of this flickering stimulus was 16 s and alternated with blank fixation periods (also 16 s); this cycle repeated twenty times during an individual scan.
Raw data were converted with the following parameters: no blank subtraction, subtract baseline and average over cycle range according each case.
Blank measurements consist of 1 block of 10 cycles (8 s) and sample and standard measurements consist of 2 blocks of 20 cycles of 8 seconds each.
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