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The blank concentrations which were recalculated to the sample amount used for the extraction are listed in Appendix 1 (Table 4).
Laboratory 2 blank concentrations for all PBDE congeners were under the LOQ < 0.1 ng/g lipids.
The net concentration was calculated by subtracting detectable laboratory blank concentrations within each batch.
Blank concentrations were below the limits of determination for Cr VI) and for total Cr.
Blank concentrations were only subtracted from Laboratory 1, and the corrected values are presented in Table 1.
Relatively high blank concentrations of 2,4,6-TBP are reasonable because this compound is the most common TBP isomer.
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Note that since the log transformation of blank concentration does not exist, in our analysis, we used only the spiked-in concentration levels, which are greater than zero.
The relatively higher blank concentration for the mother lends some uncertainty to the precision of her September measurement, but all of the September BDE-209 values were sufficiently above background to be considered valid.
The limits of detection (LODs) were calculated as standard deviations (SDs) of the method blanks analyzed in parallel with the unknowns after subtracting the average blank concentration or as the instrumental LOD in the absence of a detectable blank level (Additional file 1).
Limits of detection (LOD) were calculated as the highest of two methods: (i) 3 times the standard deviation of the method blank samples and (ii) as the lowest point in the calibration curve having a signal-to-noise ratio greater than 3 (primarily for analytes with low to no detectable method blank concentration).
All release data presented are normalized to the dry mass of the leather, mg/kgleather (1 mg/kgleather corresponds in this study to 0.02 mg/l for 1 g of sample), normalized to the leather geometric surface area, mg/cm (1 mg/cm corresponds in this study to 20 mg/l for a 1-cm sample), and as average values of triplicate samples with the corresponding blank concentration, if positive, subtracted.
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