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(A) Representative pre-operative, as well as week 1 and week 3 post-operative slit-lamp images of rabbits in Group A treatment group where DM of rabbits were removed before receiving TE-EK grafts; Group B Controls where the DM of rabbits were removed; and Group C Controls where DM of rabbits were removed and a blank carrier inserted.
The synthetic parameters of the blank carrier (CCNBs) were similar to the ECCNBs sample.
The blank carrier CCNSs were prepared without the addition of etoposide, and other experimental parameters were similar to the ECCNSs sample.
The cells were used for transfection when the cell reached 90% confluency and were assigned to either the RKIP-3xFLAG group (fusion carrier group), the 3XFLAG group (blank carrier group), RKIP group (which was used to validate whether 3xFLAG affects the expression of RKIP), or the blank group.
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In order to rule out the nonspecific bound proteins, three experiment groups were designed as pcDNA3.1-RKIP-3xFLAG, pcDNA3.1-3xFLAG (blank-carrier) control group, and the blank control group.
The blank-carrier control pcDNA3.1-3xFLAG without RKIP fusion is a good, effective, and comparable control because the relative literature [ 18- 26] has been reported that 3xFLAG coupled its specifically effective antibodies is an efficient and high-performance system to purify flag-tagged protein and its interacting partners.
The proteins identified from the blank-carrier control group and from the blank control group were regarded as non-specific proteins, and were removed from the protein list identified from the pcDNA3.1-RKIP-3xFLAG test group to rule out the non-specifically binding proteins of RKIP.
Cytotoxicity screening of blank drug carriers against mammalian cells, L-929 fibroblast cells, showed that these drug carriers exhibited no cytotoxicity against cellular system which further emphasized these drug carriers as a potential candidate for biocompatible materials.
When you see "100 MB for (fill in the blank for the carrier's charge)," be mindful that this isn't much data.
A blank 100 mm carrier wafer was spin-coated with PDMS curing agent for 30 s at 6000 rpm.
Following preparation, 100 mL of blank nanostructured lipid carriers (NLCs) and thymoquinone-loaded nanostructured lipid carriers (TQ-NLCs) was synthesized.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com