The change in absorbance at 574 nm compared to a PBS blank was assessed using a microplate specrophotometer.
All experiments were performed first with a blank to assess whether the red color of the extract influenced the test readings.
In the field, QC samples include field blanks to assess whether samples have been contaminated in the field; field spikes to assess whether the analyte concentration changes during collection, shipping, and handling; and field duplicates (or replicates) to assess the variability of the concentration in the matrix.
The changes in morphology of B16F10 cells after the treatments of 5-FU solution, 5-FU-loaded CP/GMS SLNs, and their corresponding blanks were assessed under the inverted-light microscope.
Digestion and column blanks were assessed by passing a known amount of Mo spike through the digestion and column separation chemistry, and were typically less than 3 ng, based on repeat analyses.
The calibration blank served to assess any background carryover in the ion chromatographic system.
Fifty microliters of the sample mixed with 1 ml 0.1 M Tris, 10 mM EDTA pH 8.2, constituting the blank reaction, was assessed at 412 nm (UVIKON, Kontron Instruments, Milan, Italy).
On sampling days when intervention occurred, we collected a blank BioCell to assess fungal spores that could have been on the collection media before sampling, that were introduced in the moments before starting and after stopping the pump, and during sample transport.
was assessed in blank experiments with Ar-saturated solutions and no added N source (runs 1, 9, 17, 25, 37, 43, 49, 54, 60, 66, 72, 78).
Pain was assessed using blank body manikins, a standard data‐capture method used in postal surveys.
Typically, the average of up to 500 readings was used for assessing the blank signals.
