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Thiobarbituric acid was then added and samples boiled for 15 min. The pink reaction product was extracted by butanol and its absorbance determined at 550 nm using a reaction blank as reference.
The absorbance of the newly formed mixture was immediately measured at 340 nm by using a blank as reference containing 1000 μL of PBS, 50 μL of Potassium Iyodide (KI), and 100 μL of acetic acid.
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Figure 7(b) shows the number of donors with statistically significant proliferative responses using a blank control as reference.
Flow cell 1 was left blank as a reference.
Flow cell 1 was left blank as a reference, while flow cells 2 4 were immobilized with DNA by manual injection of DNA stock solutions (flow rate of 1 μL/min) until the desired amount of DNA response units (RU) was obtained (320 330 RU).
For quality control of the analysis of mercury method blanks as well as internal reference material being monitored by means of a control chart were checked.
One flow cell left blank was used as reference.
The samples were cut in small pieces and tested in duplicate; (v) control samples: BC and CV fibers without the addition of Cu were tested as blank reference, while as internal reference of the method the bacteria growth was tested on flasks only containing inoculated Broth media.
The samples were cut in small pieces and tested in duplicate; control samples: BC and CV fibers without the addition of Cu were tested as blank reference, while as internal reference of the method the bacteria growth was tested on flasks only containing inoculated Broth media.
20 tablets of each drug were used for the photostability test: 5 tablets were not radiated in order to use them as reference (blank), while 5 tablets were exposed inside the marketing pack (cardboard box), 5 tablets were exposed inside the primary pack, 5 tablets were exposed by removing all packaging, after a fine grinding and thin layering on glass plate.
A sample with myoglobin (without valine as the N-terminal amino acid) from horse skeletal muscle was added as blank reference.
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