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Group D: blank ANPs.
Both blank ANPs and gemcitabine do not show significant difference compared with the control at the proliferation index (p > 0.05).
The blank ANPs were prepared using the same procedure as that for the drug-containing nanospheres but without the addition of gemcitabine.
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In addition, Ki-67 protein immunohistochemical assay indicates that the proliferation index of tumor cells in 110-nm GEM-ANP (36.4 ± 8.1%), 406-nm GEM-ANP (25.6 ± 5.7%), and gemcitabine (38.4 ± 9.4%) groups are lower than that in the blank ANP and control group, with significant difference (p < 0.05).
Control mice received 200 μL of saline, while blank mice were treated with unloaded ANPs.
DLS assay showed that the hydrodynamic diameter of the polyplexed blank TPGS-b- PCL-ran-PGA) NPs (ANPs) was approximaTPGS-b- PCL-ran-PGATPGS-b- PCL-ran-PGAe doceTPGS-b- PCL-ran-PGA-(PCL-ran-PGA) and doceTPGS-b- PCL-ran-PGA-(PCL-raNPsGANPsEI-coated washout pDNapproximatelyproximately 215 anm approximately 270 nm, respectively (Figure 2A).
Blank stares.
Those blanks really are blank.
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