Briefly, leaf blade samples were fixed with glutaraldehyde solution and then osmium oxide.
The effect of sample sizes in this comparison was minimal as stem samples longer than 5 mm or leaf blade samples >5 mm showed similar INT, as detailed below.
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This relationship held true among almost all O. sativa accessions, held for all leaf blades sampled and for the leaf 6 sheath and also held true for O. glaberrima.
A 1-3 mm2 spot from the filter paper was excised using a fresh scalpel blade per sample and put into a 1.5 ml pre-labeled tube at room temperature.
b Expression profile of NRGs in each module in various organs and tissues at different developmental stages based on 143 microarray data previously obtained by Sato et al. (2011) from leaf blade (6 samples), leaf sheath (4) root (4), stem (3), panicle (3), anther (3), pistil (3), lemma and palea (6), ovary (4), embryo (5), and endosperm (5).
Tissue blocks were macro-dissected using a safety blade when samples were less than 70% enriched with tumoral cells.
Unfortunately, the blade contaminates the sample surface with Cu and Sn, which is difficult to prevent or remove.
The continued rotation of the blade causes the sample to be sealed into the WCE.
