Since C fibers are the nociceptors associated with chronic pain, bladder sections were stained with isolectin B4, a marker for C fibers that stains most bladder nociceptor afferents [23], [24], [25].
Tissue microarrays with primary and metastatic tumors and normal bladder controls were stained with a commercially available anti-FGFR3 antibody (Santa Cruz, Dallas, TX, clone B-9, SC-13121).
As positive control for COX-2 and PPAR γ staining, formalin-fixed paraffin-embedded sections of colon carcinoma and urinary bladder carcinoma were stained by the same procedure, respectively.
The cells were cultivated in fresh medium after every treatment and harvested on the fourth day for RNA extraction. 2 μm slides of formalin fixed paraffin embedded (FFPE) bladder tissues were stained with ST6GAL1 monoclonal antibody LN1 clone with 1 100 dilution (MAB6959, Abnova, Walnut, CA, USA).
And then he turned and began to claw up the bricks, scratching and springing, his paws scraping and his ugly little tail jerking, and at the same time letting go his bladder until the bricks were stained with hot, terrified urine.
Bladder sections from all groups were stained with H&E and evaluated by an observer (blinded as to the treatment) for histological changes.
Bladder cancer and healthy tissue samples were stained with NT4-biotin, followed by streptavidin-FITC.
Paraffin-sections from 150 newly diagnosed bladder tumours (Ta/T1 = 97; T2–T4 = 53) were stained for cyclin D1 using immunohistochemistry and a cyclin D1 index assigned.
Slides were stained immunohistochemically for AR.Androgen receptor was positive in 95% of prostate carcinomas (n=230), but 19% of invasive urothelial carcinomas of the bladder (n=190) and 33% of non-invasive bladder urothelial carcinomas were also AR positive (N=107).
Prepared sections were stained with HE staining, Masson staining, and toluidine blue staining.
