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At 65 min after the injection, mice were sacrificed and blood, brain, muscle, liver, kidney, pancreas, BAT, WAT and bladder were collected for 18F-FDG determination.
The eggs laid in each swim bladder were collected and suspended in a cap filled with 40 ml tap water.
At week 20 blood and bladder were collected and checked for the presence of urothelium lesions and tumors, markers of inflammation, proliferation, and redox status.
Spleens and blood were collected from all bats, and other organs (liver, kidney, lung, brain, intestine, trachea, and urinary bladder) were collected from 10 bats.
Fasting blood and urine samples (morning after first empty of the bladder) were collected for both primary and secondary outcome measures.
Thirty-four tumor samples of the urinary bladder were collected at public slaughterhouses of Marmara and Black Sea Region (Turkey) (Bafra, Coskun, Bartin) from 3- to 5-year-old castrated male water buffaloes daily grazing on fern-infested lands.
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Anesthetized male Sprague-Dawley rats received either saline or substance P (s.c)., bladders were collected 1 hour after treatment and processed for histology or protein/mRNA.
Postsacrifice at 3 mo, bladders were collected and processed for immunohistology.
The bladders were collected and fixed in 4% neutral paraformaldehyde for 1 day.
The rats were transduced at an MOI of 10 for 2 hours, drained, and washed with normal saline on days 1, 3, and 5. On day 7, rat bladders were collected and frozen specimens were embedded in Tissue-Tek O.C.T. compound (Miles Scientific, Elkhart, IN).
Bladder cancer tissues and paired adjacent normal bladder tissues were collected.
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