Using CFC assays, we found that compared with the EtOH-treated controls, E2 stimulation increased the frequency of luminal progenitors by 13%, which was completely attenuated in the presence of ICI (Fig. 2C, black bars, input luminal progenitors as compared with output progenitors) and exhibited a twofold increase as compared with the input controls.
The exhibition book deals with Meroe as if it had been one nation modeling itself on Egypt, with some black African input.
Also, we observed that placing the luminal progenitors in matrigel cultures with i3T3 cells plus EtOH significantly increased the frequency of the progenitors (Fig. 2C, black bars, input as compared with the ethanol controls), which indicates that either fibroblasts or ethanol alone could increase the frequency of luminal progenitors and that estrogen may not act directly on luminal progenitors.
(C ) Cumulative distributions of excess noise levels of synthetic promoters (red) and native promoters without known regulatory inputs (black), with one known regulatory input (green), and with two or more known regulatory inputs (purple).
The black-box input-output transfer function of interconnected networks (see Figure 1A-C) is studied for different noise entities.
Second, the black-box input-output function of a two/three-module network was predicted from the knowledge of the transfer function of individual modules, identified in the presence of noise.
As a last study involving the black-box input-output transfer function of a three-module network, we compared the black-box function predicted by using the individual modules identified from data generated with different noise entities affecting OUTPUT1, and the black-box function simulated by using different noise entities affecting OUTPUT1 and OUTPUT2.
We performed simulated experiments where: i) the transfer function of each single module was identified from population-averaged values (as performed in the previous section) and ii) the identified transfer functions were used to predict the black-box input-output function of the interconnected network.
Second, we have tested the identification of a black-box input-output function predicted by interconnecting the three genetic modules that were previously characterized in silico, in the presence of noise, via central tendency-based measurements to identify their transfer function.
In Fig. 1, black circles are input signals by which the automotive engine behaves normally, and red circles indicates input signals by which the automotive engine does not behave normally.
