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Thus, each q-gram is encoded as an unsigned integer with two bits per nucleotide.
Previous research [ 1] show that for the sequence of a human genome it is hard to achieve compression rate significantly better than a trivial two bits per nucleotide.
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By making use of a 2-bit per nucleotide coding strategy to speed up performance, every 25-bp unique spacer is queried against each read allowing up to one mismatch (Ioerger et al., 2009).
Simple satellite sequences appear to be more highly conserved than coding regions of the genome, on a per nucleotide basis.
A naive representation (2 bits per base, packed), would use 50 bits per 25-mer, or about 26 GB.
As said, a single genome in its compact encoding (2 bits per base) seems almost incompressible.
Sequence data are stored in compressed format with 2 bits per sequence base using NCBI2NA encoding.
The scale bar corresponds to the expected number of changes per nucleotide position.
While these differences contribute only a 0.1 0.4 bit difference to the R i per nucleotide, the cumulative effect of multiple differences within this window can lead to significant differences between the acceptor R i values.
The average number of between-group nucleotide differences per nucleotide site was designated the nucleotide divergence (d).
In addition, the average numbers of insertions per nucleotide per read and the average number of deletions per nucleotide per read were 0.003% and 0.015%, respectively.
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