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This conclusion is also important in neuronal replacement experiments, where BrdU and another cell birth marker are given, with relatively long intervals between them.
The main aim of this study was to determine whether treatment with the cell birth marker BrdU affects subsequent cell divisions and the recruitment of neurons that differentiate from these divisions into various brain regions.
We thus injected birds with BrdU and either one or three months later treated them with another cell birth marker, [H]-thymidine (groups 1 M and 3 M, resp).
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This can be highly relevant to experiments which examine the dynamics of neuronal replacement by using two birth markers.
The present study, which indicates that BrdU does not have a long-term effect on the future functionality of neurogenesis, thus has relevance when planning experiments which use two birth markers, for example, when examining the dynamics of neuronal replacement.
To provide evidence for neuronal replacement, we used two cell birth markers in the same birds: BrdU, to label neurons born 1 or 3 months prior to social manipulation [ 32], and [H]-thymidine, to label neurons born during the 5 days immediately before the manipulation (unpublished data).
Based on predefined pregnancy and birth markers [ 10], women of childbearing age (11-50 yeats at cohort entry) were screened for pregnancy within 270 days prior to cohort entry up to 270 days after the estimated end of the last rivaroxaban treatment episode.
For example, increased risk of breast cancer correlated with twin dizygotic birth, a marker of high estrogen exposure (Braun et al. 1995), and preeclampsia, a marker of low estrogen exposure, was associated with lowered risk (Innes and Byers 1999).
The timepoint of first injection was used to additionally inject the birth date marker BrdU in a similar injection volume for all four groups.
Given the heterogeneity of pathways leading to spontaneous preterm birth, multiple markers increase sensitivity of prediction by combining risk predictors that address diverse causes of spontaneous preterm birth [ 31].
If so, then this effect was then reflected in the unexpected results that we obtained when we later treated the same birds with the second birth-date marker, [H]-thymidine, and recorded [H]-thymidine+ neurons.
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