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Samples from groups of 5 birds were pooled.
Subsequently, samples from these 80 birds were pooled in 8 pools of ten birds.
To account for the well known effects of MHC blood type on resistance to MD [ 34- 36], birds were pooled within B blood groups.
Encounter histories of birds were pooled into four time categories, comprising September – May during 2005/06, 2006/07, 2007/08 and 2008/09.
As no differences were evidenced between these two groups (e.g. mean proportions of responsive sites: raised in isolation = 92.10±1.32 and pair raised = 93.37), data from these three birds were pooled (socially deprived birds: SD).
Similar(55)
Therefore, the results from sham operated and untreated birds are pooled below, and these birds are henceforth referred to as 50NS-H group.
Equal amounts of RNA from each bird were pooled.
Tissues from each individual bird were pooled and homogenized in 2 mL of BA1 supplemented with 20% fetal bovine serum.
All of tissue punches from one nucleus of one bird were pooled, flash frozen in dry ice, and stored at –80 °C until processed for microarray hybridization.
To confirm the infection status of birds, approximately 25-mg pieces of brain and kidney from each bird were pooled in microcentrifuge tubes and stored at –80°C before shipment to the National Microbiology Laboratory.
For each tissue 1 μg of RNA from each bird was pooled, i.e. to make 8 tissue pools, each containing 10 μg of RNA.
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