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All birds were continuously monitored for clinical symptoms and blood samples were collected at 1, 2, 4 and 7 weeks after LPAIV inoculation.
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Moreover, throughout the experiments, all birds were maintained continuously on the antibiotic drug tylosin tartrate (0.3 mg/ml).
Birds were placed individually in sound attenuation chambers for 5 days while their songs were continuously recorded.
The flies were continuously active.
Subjects were continuously monitored by trained staff.
Temperature and ECG were continuously monitored.
were continuously measured.
Samples were continuously collected.
were continuously infused.
ECGs were continuously monitored.
Clade 2.3.2 viruses might spread over an extensive area, similar to clade 2.2 viruses, because clade 2.3.2 viruses are widespread among wild birds and have been continuously evolving in the regions where subtype H5N1 viruses are endemic (9 ).
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