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Ethical approval for sampling ticks from birds was obtained from the Malmö/Lund animal research ethics board.
After enrichment for 1 Mb around the QTL region, >500 × coverage for the QTL region in each of the 10 birds was obtained.
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The birds were obtained from different local breeders.
Blood samples of wild birds were obtained at different European localities from southern Spain to Sweden, at migration stopover sites, and in European and African wintering areas.
The birds were obtained from local suppliers and were kept in the laboratory aviaries with unrestricted access to food and water, temperature between 20°C and 25°C, and natural light/dark rhythm.
Because the kestrel colony where these birds were obtained has a history of West Nile virus (WNV) activity and infection with that virus could confound our analyses, we also screened the sera for WNV antibodies by PRNT90.
Methods Public reports of dead birds were obtained from the DBSP.
Birds were obtained from the Massachusetts Institute of Technology zebra finch breeding facility (Cambridge, Massachusetts).
Birds were obtained commercially when possible or as nonreleasable injured birds (raptors only), or from the wild (Table 1).
In Australia in 2001 2002, isolates from birds were obtained from feces of clinically healthy domestic and trapped wild birds (8 ).
The correct ethical approvals/permits to sacrifice birds were obtained for both species included in this study.
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