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After 24 hr of incubation in darkness at 25°, a mycelia plug was cut with a No. 6 cork borer (12-mm diameter) and transferred into a 125-mL flask with 50 mL of Bird medium containing 1.8% glucose.
A total of 1 × 10 of 1-week-old conidia were inoculated into a 10-cm Petri dish with 20 mL of Bird medium (Metzenberg 2004) containing 1.8% glucose (for the vvd promoter) or Vogel's salts with 2% glucose (for the ccg-1 promoter).
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Unmanipulated birds (medium allostatic load) also experienced weight loss but had normal healing rates, while birds with blocked corticosterone (low allostatic load) had minimal weight loss and normal healing rates.
Table 1 Sample size in different categories of poultry production system Free-range (<=50 birds) Small-scale (51 999 birds) Medium-scale (1000 5000 birds) Large-scale (>5000 birds) All households Anambra 163 34 5 1 203 Lagos 121 49 23 15 208 Kano 95 66 22 17 200 Source: Field Survey, 2010 and 2014.
Growth in race tubes was analyzed using Bird's medium without carbon source (control) and with 1% of sucrose or carboxymethylcellulose (CMC) as carbon source.
Conidia were harvested with water and inoculated into 50 ml of Bird's medium [ 91] with 2% (w/v) Avicel cellulose (Sigma) as sole carbon source in 250 ml Erlenmayer flasks at a final concentration of 106 conidia/ml.
Mr. Wahlgren said a shipment usually included one very large bird, one medium-size bird and three small birds.
The ibis form an even more homogeneous group, birds of medium size with markedly downcurved, slender bills; the spoonbills, of the same family, differ in this respect, as the name implies.
The study results show that the key land use types for protecting endangered species of birds are medium grassland, tidal flat and pond landscapes.
Ungulates, birds, small-medium vertebrates, and carnivore collision locations were associated with road-side vegetation and other features such as salt pools.
An adult's diet is largely fish, caught by sweeping the snout sideways and snapping at the prey, but also includes shrimps, crabs, frogs, snakes, birds and medium-sized mammals.
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