Five proteins in this network (DSK2, HSP82, HSA1, BiP, and SMK1) are significantly and differentially expressed.
These include Armet, ATF3, BiP, CHOP, Dnajb1 (DnaJ (Hsp40) homolog, subfamily B, member 1), Hmox1, HSP27, among others.
Based on a rigid ligand 3,5-bis imidazole-1-yl pyridine (bip), three new low-dimensional Zn(II)/Cd(II) coordination polymers [Zn(bip)(Cl)2]n (1), [Zn(bip)(Cl)2]n (2) and [Cd(bip)(p-bdc)0.5(NO3,5-bis imidazole-1-yl pyridine4-benzenedicarboxylic acid) have been successfully obtained.
Furthermore, crosslinking of thiouridine-labeled BiP IRES-containing RNA to cellular proteins identified the specific binding of two proteins, p60 and p95, to the 3'half of the BiP 5'NCR.
Kim and colleagues and Kwon and colleagues reveal that amino-terminal arginylation of BiP promotes its targeting to autophagy adaptor p62 and subsequent lysosomal degradation of BiP, p62 and associated cargo.
Remarkably, in bifunctional human FICD, the autoinhibitory glutamate is critical for deAMPYlation of the BiP chaperone18, a reaction whereby FICD contributes to the activation of BiP under ER stress conditions.
Three of the down-regulated genes, GRP78 (BiP), Dad1, and mitochondrial aspartate aminotransferase have been previously shown to be directly androgen regulated.
TUDCA reconciled obesity-associated decrease in SERCA activity and expression, and increase in serine phosphorylation of IRS, total and phosphorylated cJun, ER stress markers Bip, peIF2α and pPERK.
Resident ER proteins Sec61, BiP (Kar2) and Shr3 were not included in the complexes, indicating that the COPII components bound specifically to vesicle cargo.
A Western blot analyses of whole cell lysate (WC), postnuclear supernatant (PNS), and the 12 fractions using antibodies against phogrin, β-granin, Bip, TGN46, and GAPDH.
