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Furthermore, surface biotinylation experiments indicated that the relative number of membrane expressed 2-aminoethyldiphenyl borate -activated TRPV2 channels were not altered by citral treatment (data not shown).
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Cell surface biotinylation results indicated that Orai1 channels in the plasma membrane were assembled from intact hexameric polypeptides and not from truncated protein products.
The gross comparison of biotinylation patterns indicated that the complement of surface-exposed proteins from the POS and HMPOS cell lines appeared similar, while the surface proteome of the CnOb cell line appeared to be markedly different.
Our confocal microscopy and biotinylation experiments strongly suggest that only the very small fraction of METΔ7 8 that is cleaved reaches the cell membrane, but this fraction is insignificant with respect to the auto-active component in the cytosol.
The presence of intracellular protein biotinylation indicated that biotin-spm is cell permeable, and that Gspm-biotin molecules have formed mixed disulfides with protein cysteine residues.
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