The avidin-biotin complex was visualized using 3.3' diaminobenzidine (DAB) peroxidase substrate (Vector Labs).
HIV-1 core antigen was visualized using biotin-streptavidin-alkaline phosphatase complex and Vector Red (Abcys), as an AP substrate.
Primary antibody binding was visualized using biotin-labeled anti-mouse IgG in the Mouse on Mouse detection kit from Vector Labs (Burlingame, CA, USA) PK-22000).
Myelination was visualized using avidin-biotin-peroxidase complex method with diaminobenzidine as the chromogen as described in the immunochromogenic staining.
Antibody was visualized using the TSA biotin system (Perkin-Elmer, Waltham, MA, USA) followed by streptavidin-conjugated Alexa Fluor 549 (1 : 1000, Invitrogen).
Bound antibody was visualized using the avidin-biotin-peroxidase complex (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA, USA).
Immunostaining was visualized using the avidin-biotin system (Vectastain; Vector Laboratories, Burlingame, CA) and 3,3'-diaminobenzidine (Sigma) as the chromogen.
The primary antibody was visualized using a streptavidin-biotin-peroxidase (ABC) kit (DAKO, Carpinteria, CA) with diaminobenzidine tetrahydrochloride (3,3'-diaminobenzidine) as the chromogen.
Immunolabeling with the primary antibody listed in Table 1 was visualized using the streptavidin-biotin-peroxydase method, as previously described [8].
Immunolabeling with the primary antibodies listed in File S1 was visualized using the streptavidin-biotin-peroxidase method as previously described [15].
