Sentence examples for biotin was saturated using from inspiring English sources

For antigen retrieval (CD31 only, not necessary for VEGF) the section were microwave treated in 1 mM EDTA at pH 8 for 10 min and allowed to cool for 20 min. Endogenous biotin was saturated using a biotin blocking kit (Vector Laboratories).

Endogenous biotin was saturated by biotin blocking kit (Vector).

Endogenous biotin was saturated with a biotin blocking kit (Vector Laboratories).

For all patients the contrast-enhanced T1-weighted images were fat saturated using spectral saturating technique.

Another set of tips was saturated with MBP-biotin and the measurements were then repeated for all Hfq and Hfq RNA concentration series.

Avidin-kodecytes will not self-aggregate provided all (most) biotin residues are saturated with avidin, this can be achieved by using avidin in excess.

ECS were saturated with O2 before using.

The amount of biotin was determined using the following equation: V biotin = 1, 000 × m PLGA M W biotin M W PLGA M biotin C biotin Open image in new window (2).

Excess biotin was removed using a 0.7-ml spin-column.

When biotinylated secondary antibody was used, endogenous biotin was inhibited using an avidin/biotin blocking kit from Vector.

Unlabeled EGF (Roche, Manheim, Germany) was biotinylated using sulfo-NHS-LC-biotin (PIERCE), and excess biotin was removed using Zeba Desalt Spin Columns PIERCEE).