Sentence examples for biotin was removed using from inspiring English sources

Excess biotin was removed using a 0.7-ml spin-column.

Unlabeled EGF (Roche, Manheim, Germany) was biotinylated using sulfo-NHS-LC-biotin (PIERCE), and excess biotin was removed using Zeba Desalt Spin Columns PIERCEE).

Excess biotin was removed using a 2 ml desalting column.

Free biotin was removed using microconcentrator (PALL Gelman Lab., Wien, Austria) or gel filtration.

After defined time for endocytosis, cells were rapidly returned to 4°C, and surface-exposed biotin was removed using the membrane impermeable reducing agent MESNa.

After incubation at room temperature for 30 min, stop solution from the kit was added, and free biotin was removed using a spin column.

Then, excess biotin reagent was removed using a desalting column.

Unbound biotin-EGF was removed using a size exclusion column.

The majority of proteins that could be labeled with biotin-maleimide were removed using activated thiol beads (lane 4).

It includes a disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT and which prevents intracellular protein capture due to the strongly reducing environment of the cell.

CD45+Ter119+ and CD31+ cells were removed using the EasySep biotin selection kit (StemCell Technologies) to obtain Lin− cells.