Sentence examples for biotin was inhibited using from inspiring English sources

When biotinylated secondary antibody was used, endogenous biotin was inhibited using an avidin/biotin blocking kit from Vector.

CI was inhibited using 2 μM rotenone.

After pretreatment in a microwave oven, endogenous peroxidase was inhibited by 3% hydrogen peroxide in methanol for 20 min, followed by avidin-biotin blocking using a biotin-blocking kit (DAKO, Germany).

The amount of biotin was determined using the following equation: V biotin = 1, 000 × m PLGA M W biotin M W PLGA M biotin C biotin Open image in new window (2).

Excess biotin was removed using a 0.7-ml spin-column.

Unlabeled EGF (Roche, Manheim, Germany) was biotinylated using sulfo-NHS-LC-biotin (PIERCE), and excess biotin was removed using Zeba Desalt Spin Columns PIERCEE).

Endogenous biotin was blocked using an endogenous biotin blocking kit.

First, endogenous biotin was blocked using a sequential avidin biotin treatment (Biotin blocking system, Dako, Trappes, France).

Excess biotin was removed using a 2 ml desalting column.

Biotin was visualized using streptavidin-biotinylated alkaline phosphatase complex (DakoCytomation).

The molecular interaction between biotin and an anti-biotin antibody was shown to be inhibited by 41% due to the presence of biotin in a sample.