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Interestingly, several polyketide synthases (PKSs) and multiple fatty acid desaturases potentially involved in DHA biosynthesis were identified in the C. cohnii transcriptome, most of which were significantly up-regulated during lipid and DHA accumulation, implying that C. cohnii might utilize a combination of PKS systems and desaturase steps for DHA biosynthesis.
Another interesting observation is that, although the genes for ectoine biosynthesis were identified in P. stutzeri TAIHU as described earlier, the operon responsible for the degradation of ectoines (doeABCD) was found absent in P. stutzeri TAIHU, but present exclusively in M. sp. TAIHU (Fig. 4c).
Complete pathways for purine and pyrimidine biosynthesis and several components of the salvage pathways for purine and pyrimidine biosynthesis were identified in B. hyodysenteriae.
Notably, many genes involved in triterpene saponin biosynthesis were identified in this study.
Genes encoding enzymes involved in chlorophyll degradation and flavonoid biosynthesis were identified in the transcriptome dataset.
Most enzymes involved in the lipid biosynthesis were identified in the tree peony seed transcriptome.
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Another gene cluster involved in LOS biosynthesis was identified in the H. parasuis SH0165 genome, which also has a low G+C content (28.4%), indicating that this DNA region may originate from a species other than H. parasuis.
One set of single-copy genes involved in tetrapyrrole biosynthesis was identified in the A. platensis genome.
Recently, a gene cluster encoding a group of biosynthetic genes required for cercosporin biosynthesis was identified in Cercospora nicotianae, a foliar pathogen of tobacco [ 15].
A total of 27 unique genes involved in tocochromanols biosynthesis were identified as DEGs in our experiment (Table 2 and Additional file 5).
The majority of genes governing fatty acid biosynthesis were identified as being overexpressed in nitrogen limited cells as shown in the global metabolic pathway level and fatty acid biosynthesis module (Additional file 6).
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