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AtIPT genes involved in Ck biosynthesis were found to be up-regulated in those transgenics while all AtCKO genes were down-regulated except At5g21482, coding for a putative cytoplasmic enzyme.
Notably, a total of 65 enzymes involved in lipid biosynthesis were found to be acetylated.
Notably, a total of 65 enzymes involved in lipid biosynthesis were found to be acetylated (Fig. 8a; Additional file 8: Table S7).
Through the genome analysis, most of the genes involved in the cobalamin biosynthesis were found to be absent in the genome of S. sp. PCC 7002 (Fig. 4a), that explains the inability of this organism to synthesize cobalamin de novo.
A large number of enzymes associated with TAGs biosynthesis were found to be acetylated (Fig. 8a), supporting the notion that lipid biosynthesis might be strictly regulated by lysine acetylation.
However, few pathways such as tetrahydrofolate biosynthesis were found to be variable and could be contributing to adaptation towards virulence such as antibiotic resistance.
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Thus, BS biosynthesis was found to be growth-associated which is in accordance with earlier reports43,44.
Previously, monoterpene biosynthesis is found to be developmentally regulated (Hsiao et al. 2008).
YUCCA2 involved in auxin biosynthesis was found to be up-regulated by glucose.
In contrast, the expression of delta-aminolevulinate synthase (alas2), which is the enzyme required for the first step in heme biosynthesis, was found to be decreased from 26 hpf (n = 41 of 41; 100%) to 32 hpf (n = 50 of 54; 93%) (Figure 3B, e, f, e', f', data not shown for 26 hpf).
The biosynthesis was found to be via farnesyl diphosphate.
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