A pathway for stilbene biosynthesis was constructed in Escherichia coli with 4-coumaroyl CoA ligase 1 4CL1) from Arabidopsis thaliana and stilbene synthase (STS) cloned from Arachis hypogaea.
Transconjugants harboring cyanobacterial expression vectors that carried the pha genes for PHB biosynthesis were constructed.
Seven pathways of vanillin biosynthesis were constructed, namely, glucovanillin, glucose, cresol, capsaicin, vanillyl alcohol, tyrosine, and phenylalanine pathways.
In addition, 21 precursors related to vanillin synthesis were identified, and 7 pathways of vanillin biosynthesis were constructed, including glucovanillin, glucose, cresol, capsaicin, vanillyl alcohol, tyrosine, and phenylalanine pathways.
In-frame deletions that removed more than 90% of the coding sequence and were designed to not interfere with expression of downstream genes in the same operon were constructed in these and other genes involved in LPS biosynthesis.
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LacZ translational fusions were constructed in pUC19.
In our previous study, an arachidonic acid biosynthesis pathway was constructed in Yarrowia lipolytica, and interestingly, the engineered strain achieved higher biomass production without metabolic burden.
C35 carotenoid biosynthesis pathway has been constructed in E. coli as described.
