Interestingly it should be noted that another pathway related to steroid production (sterol biosynthesis) was also enriched between in vivo and in vitro embryos.
In Arabidopsis, genes involved in flavonoids biosynthesis and circadian rhythm pathways were also enriched in chilling up-regulated DEGs.
Other biological processes such as ketone biosynthesis, pigment metabolism, and reductive pentose phosphate were also enriched in Vagad during the irrigated condition.
Again, there was some overlap between responses as the anisomycin-upregulated genes were also enriched for components of RNA biosynthesis pathways, whereas all the stresses showed some enrichment for regulators of cell migration, proliferation and apoptosis.
These two species were also enriched for thiamine (vitamin B1) biosynthesis, suggesting potential similarity in dietary supplementation in Xiphinematobacter and A. muciniphilia from the human colon.
In addition to signaling pathways, cellular metabolism functions were also enriched in the modules, including macromolecule biosynthesis (module 0), catabolism (module 1), RNA metabolism (module 2), and nucleotide metabolism (module 9).
Interestingly, these clusters were also enriched in genes related to lipid biosynthesis and storage such as phosphatidylinositol transfer protein, delta 6-fatty acid desaturase/delta-8 sphingolipid desaturase, delta12-fatty acid dehydrogenase desaturase, Fatty acid biosynthesis I, oxysterol binding protein or patatin.
A20 overexpressing liver were also enriched for genes involved in amino acid, fatty acid, steroid, and cholesterol biosynthesis, indicating that A20 primarily modulates the liver biosynthetic and metabolic processes.
In addition, some pathways involved in genetic information processing were also enriched, such as protein processing in endoplasmic reticulum, DNA replication, aminoacyl-tRNA biosynthesis, ribosome biogenesis in eukaryotes, and RNA transport.
