In addition to TAG metabolism, transcriptional regulation of phospholipid biosynthesis is also affected in mutants impaired in AdoHcy catabolism.
As discussed, we have observed that the combined effect of exogenous gliotoxin exposure and gliT deletion has a significant impact on secondary metabolism, despite laeA expression being unaffected, and therefore it is interesting that siderophore biosynthesis is also affected.
Core genes of the fatty acid and lipid biosynthesis pathways were also affected, and in this case they were up-regulated in corollas of ir- hd20 plants (Table 1).
Taking into account the results of TEM, where we observed the accumulation of many starch granules (to different degrees) in chloroplasts after IM-enantiomer exposure, we speculated whether carbohydrate biosynthesis or metabolism were also affected enantioselectively.
Several transcripts related to cholesterol biosynthesis and transport were also affected (Hmgcr, Srebf1, Bzrp, Dhcr7, Dhcr24, Apoa1, Abca1).
The expression of genes encoding enzymes involved in prostaglandin metabolism implies that the conversion from prostaglandin H2 to E2, F2 and D2 may be enhanced, while conversion to I2 may be reduced.Cholesterol biosynthesis and steroid hormone metabolism were also affected by rosiglitazone.
Genes associated with amino acid biosynthesis, especially with synthesis and fate of glutamine (RB4269) and glutamate (RB5653) were also affected.
