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The genomic regions for the biosynthesis of cell surface molecules have also significantly diverged between the two strains.
Large expansion of genes involved in the biosynthesis of cell surface polysaccharides and other antigens was also noted in these genomes [ 16, 35].
Besides flagellar functions, a large number of genes involved in several metabolic activities, iron acquisition, regulatory proteins, chaperones, and biosynthesis of cell surface components have been demonstrated to be important for this multicellular migration [ 7, 8].
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Although the absolute values of specificity are generally low (below 0.2 for most pathways), the trend exemplified above is clear: pathways of coenzyme biosynthesis and of cell surface component assembly have higher specificity than central pathways of intermediary metabolism.
The strain-specific genes were mainly involved in the biosynthesis and modification of cell surface structures such as flagella, lipooligosaccharide (LOS), and capsular polysaccharide (CPS) (Dorrell et al. 2001; Gilbert et al. 2002; Karlyshev et al. 2005).
Another situation exists with the rfb-gene cluster that differs entirely between the two strains and encodes several glycosyltransferases possibly involved in cell wall biosynthesis and the modification of cell surface properties.
On chromosome 4, a signal is detected on the PIGG gene, which is involved in the glycosylphosphatidylinositol anchor biosynthesis pathway, allowing the attachment of cell surface proteins to the cell membrane (Stokes et al. 2014).
Therefore, the modification of cell surface polysaccharide and lipopolysaccharide biosynthesis by several of these enzymes, presumably allowing diversification of cell surface features appears central for this group of organisms.
Many studies have shown that poorly differentiated tumors have reduced expression levels of cell surface HSPGs [ 35], indicating that the biosynthesis of HS PGs is reduced in these tumors.
Molecular mechanisms of capsular polysaccharide synthesis have been elucidated; genes involved in polysaccharide biosynthesis and cell surface translocation are clustered at a single chromosomal locus termed cps.
It is conceivable that the regulation of biosynthesis via formation of protein complexes among biosynthetic enzymes is faster than transcriptional regulation, and that this mode allows determining subtle changes of cell-surface type II AG structures during cell differentiation in plants.
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