In addition, the DNA biosensor showed a good response towards arowana cDNA, which implies that the electrochemical DNA biosensor could be used to successfully detect the arowana DNA segments.
The developed biosensor showed a good performance for detecting pirimiphos-methyl, with a limit of detection of 0.2 nM, a concentration much lower than the maximum residue limit allowed set by international regulations (164 nM).
Under the optimal experimental condition, the constructed glucose biosensor showed a good linearly relationship between the concentration of glucose and the electrochemical current, a high sensitivity of 61.78 μA/mM cm2, and a fast response time of <5 s.
Under optimum conditions, the biosensor showed a good linear relationship between the current value and logarithm of the target DNA concentration ranging from 10 to 1000 pM with a low detection limit of 6.0 pM (S/N = 3).
The biosensors show a good dynamic range, suitable for physiological monitoring.
The designed biosensor showed a very good linearity for 0.01 0.5 mM glucose.
The biosensor showed a very good analytical performance for phosmet detection, with a detection limit of 0.1 nM.
Moreover, the biosensor showed a rapid response to H2O2, a good stability and reproducibility.
Under optimal detection conditions, the constructed sensor had a linear response range of 2.8 × 10−4 M to 3.3 × 10−2 M and the detection limit was 1.8 × 10−4 M at a signal-to-noise ratio of 3. The biosensor showed an acceptable reproducibility, good stability and low interferences.
Comparing the predicted amino-acids biosensors with a list of known amino-acid auxotrophic strains (extracted from the Coli Genetic Stock Center (CGSC); http://cgsc.biology.yale.edu/) shows a good match between the two (Table 1).
