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The biosensor is specific for Tdp1 even in complex biological samples, such as human cell extracts, and may consequently find future use in fundamental studies as well as a cancer predictive tool allowing fast analyses of diagnostic cell samples such as biopsies.
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As a control to show that binding of Syt to streptavidin biosensors is specific and mediated by the N-terminal biotin tag, we incubated streptavidin biosensors with 5 mM biotin for 2 h at room temperature prior to the addition of 2.5 μg/mL N-terminally biotinylated Syt1-C2AB under the conditions described above.
Controversies of this sort have provided much of the impetus for the development of biosensors that are specific for herbicides such as atrazine, and there have been multiple attempts at using PSII-containing biomaterials in a biosensor aimed at this molecule, and herbicides from the same family.
The biosensor was determined to be specific for TGF-β1 with an experimental detection limit of 1 ng/mL and linear range extending to 250 ng/mL.
Experimental results demonstrated that the biosensor was highly specific for Pb2+ and exhibited remarkable improvements of electrochemical analytical performance.
That biosensor is also not specific for the base and can be used to detect all NDPs and dNDPs.
Here, a novel and simple surface plasmon resonance (SPR) biosensor was developed for specific and highly sensitive detection of target miRNA employing the mismatched catalytic hairpin assembly (CHA) amplification coupling with programmable streptavidin aptamer (SA-aptamer).
Regeneration of biosensor indicates whether the biosensor is reusable for a series of consecutive analyses.
The biosensor is characterized for its interaction with guanine and other nucleotides.
Based on the annihilation between the oxidation product of Fc (Fc+) and Et3N radical, a quenching-based sandwich-configuration ECL biosensor was established for sensitive and specific detection of concanavalin A (ConA).
Here, a simple electrochemical biosensor was developed for highly sensitive and specific detection of target miRNA using mismatched catalytic hairpin assembly (CHA).
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