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US-guided synovial biopsy was performed using the portal and forceps technique [ 23].
Liver biopsy was performed using a 16-gauge needle.
Percutaneous liver biopsy was performed using the Menghini needle (Hepafix kit 1.4 or 1.6 mm, Braun).
A pleural biopsy was performed using a Cope's needle and/or video-assisted thoracoscopic surgery.
Liver biopsy was performed using 18G BioPince biopsy needles (InterV-MDTech, Gainesville, Florida).
Biopsy was performed using an 18-gauge automatic cutting needle (Bard Magnum, Bard, USA).
Similar(47)
In the case of pathways 2 7, SLN biopsy is performed using either blue dye, 99mTc, or both, in order to identify the SLN.
Genotyping of the mice from tail biopsies was performed using a PCR-based method, as previously described [9].
DNA extraction of mucosal biopsies was performed using the commercially available Qiagen QIAamp Mini kit (Qiagen Crawley UK) with the following amendments.
Amplification of genomic DNA extracted from tail biopsies was performed using Taq DNA polymerase (Fermentas AB, Vilnius, Lithuania) and a program of 1 min at 95°C, followed by 30 cycles at 95°C (30 s), 60°C (30 s), and 72°C (1 min).
Immunostaining of 5 µm sections from paraffin-embeded liver biopsies was performed using a combination of the following markers, Galectin-9 (goat-anti-human Galectin-9, 1∶100, R&D Systems), CD-68 or Albumin (mouse-IgG-anti CD68 or mouse-IgG-anti Albumin, 1∶100, Abcam) in combination with DAPI for nuclear staining (1∶20,000, Invitrogen).
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