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In one patient, a core biopsy was obtained using a trephine drill (Komet Dental, Germany) in a bucco-lingual direction to harvest the tissue sample for histo-morphometric analysis.
In the past, distal duodenal/upper jejunal biopsy was obtained using Crosby-Watson capsule.
A 1-cm placental biopsy was obtained using a placental measuring gauge to ensure all biopsies were collected from the same approximate region of the chorionic foetal side of the placenta.
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RNA from tumor biopsies was obtained using the TriReagent (Gibco BRL Grand Island, New York) RNA extraction kit following the manufacturer instructions.
Total RNA from patient biopsies was obtained using an RNeasy kit on a QiaCube apparatus (QIAGEN) and served as the template (250 ng) to generate cDNA (Superscript VILO cDNA Synthesis Kit; Invitrogen).
Until the 1970s, biopsies were obtained using metal capsules attached to a suction device.
The biopsies were obtained using a Bergström biopsy needle after local anaesthesia in the ICU patients and just after sedation in the control patients.
Muscle biopsies were obtained using the percutaneous needle [55], [56] method using manual suction from the vastus lateralis under local anaesthetic (1% lidocaine) as described previously [57].
Skin biopsies were obtained using previously reported methods [ 14].
8-mm ear punch biopsies were obtained using STIEFEL biopsy punches (Schuco, Germany).
Esophageal biopsies were obtained using standard sized, Radial Jaw 4 biopsy forceps (Boston Scientific, Boston, MA, USA).
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