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When required, biopsies were thawed and a small piece of each biopsy was fixed using formalin, embedded in paraffin for histopathological confirmation of the tissue origin.
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Biopsy specimens from duodenum and the bulb were fixed in formalin.
For histological examination, colon biopsies were fixed using standard processing on formalin-fixed paraffin embedded tissue.
The lung tissue biopsies were fixed using 4% buffered formalin and embedded in paraffin for hematoxylin and eosin (HE) staining.
Biopsy specimens were fixed using buffered formalin and then embedded in paraffin.
Cells were fixed using Cytofix Cell Fixative BD Biosciencess).
A separate biopsy was fixed in neutral 4% buffered formalin and used for immunohistochemistry. Gene expressions were studied using array slides produced at the Microarray Facility at Health Enterprise Rikshospitalet-Radiumhospitalet, containing 15000 cDNA clones.
Half of each biopsy was fixed in formalin, embedded in paraffin, sectioned according to routine clinical procedures and was used for immunohistochemistry.
SSc and normal skin biopsies were fixed in formalin, embedded in paraffin and cut into 4 μm serial sections using a microtome.
The biopsies were fixed overnight in 4% neutral buffered formalin.
The biopsies were fixed overnight in 4% phosphate-buffered formaldehyde and transferred to alcohol 70%[38]].
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