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Unstained biopsy sections were submitted for polymerase chain reaction (PCR) testing in which no AFB DNA was detected with 16S rDNA, rpoB, and hsp65 primer sets, no M. tuberculosis complex DNA was detected with hsp65-amplified probe, no Mycobacterium avium complex DNA was detected with hsp65-amplified probe, and no fungal DNA was detected with 28S rDNA and ITS primer sets.
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In case of disagreement (n=13, 11.8%), sections were submitted to a rejoint evaluation.
In case of disagreement (n = 6/68, 8.8%), the sections were submitted to a rejoint evaluation.
Biopsy sections were deparaffinised using xylene and ethanol [21].
The biopsy sections were stained with hematoxylin and eosin.
Stained synovial biopsy sections were evaluated by computerized image analysis.
Frozen unfixed synovial biopsy sections were fixed with acetone.
The biopsy sections were stained with hematoxylin eosin.
All biopsy specimens were submitted for pathological examination.
Multiple biopsy samples were submitted for histologic analysis, culture, and molecular biological analysis.
All renal biopsy specimens were submitted for diagnostic purposes and proceeded using our standardized routine protocol.
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