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To test the efficacy and accuracy of SHG imaging of collagen in skin and to delineate the borders of skin cancers, unstained human melanoma biopsy sections were imaged using SHG microscopy.
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Sections were imaged immediately after incubation.
Sections were imaged as previously described.
Sections were imaged with confocal microscopy (Olympus).
Sections were imaged using Zeiss microscope.
Sections were imaged and analyzed using Image J software.
Stained synovial biopsy sections were evaluated by computerized image analysis.
In the blinded manner described, stained synovial biopsy sections were evaluated by computerized image analysis, in which the area of positive staining was expressed as a percentage of the total tissue area, for IL-15 neutralizing and non-neutralizing antibodies, IL-1α, IL-1ß and TNF mAb 2C8.
Stained liver biopsy sections were scanned with an Aperio Image-Scope Scanner (Leica Microsystems, Wetzlar, Germany).
Post-extraction biopsy sections were classified by a board-certified pathologist, and representative histology images are shown in Figure 5.
Biopsy sections were deparaffinised using xylene and ethanol [21].
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