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Frozen unfixed synovial biopsy sections were fixed with acetone.
Frozen human muscle biopsy sections were fixed in ice-cold acetone.
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MLN and spleen biopsies were frozen and 5-µm cryostat sections were fixed in acetone, blocked in 1% BSA and stained with CD8 mAb (supplemental Table S2).
For detection of β-glactosidase expression in sections, the biopsies were embedded in OCT; 10 µm sections were fixed in acetone/methanol (50/50) for 10 minutes and subsequently incubated in 1 mM X-gal for 3 hours.
Frozen sections were fixed in 10% formamide.
Sections were fixed in 4% paraformaldehyde.
Ileum sections were fixed with 4% paraformaldehyde.
Frozen sections were fixed in 10% formalin.
Sections were fixed in acetone.
Sections were fixed in formaldehyde.
Frozen sections were fixed in cold acetone.
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