Biopsy sections were deparaffinised using xylene and ethanol [21].
Stained synovial biopsy sections were evaluated by computerized image analysis.
Frozen unfixed synovial biopsy sections were fixed with acetone.
The biopsy sections were stained with hematoxylin and eosin.
The biopsy sections were stained with hematoxylin eosin.
Stained liver biopsy sections were scanned with an Aperio Image-Scope Scanner (Leica Microsystems, Wetzlar, Germany).
RMS cells in the biopsy sections were discerned by staining for myogenin (MYF4; #L026; Immunologic, Duiven, the Netherlands).
The material from the 43 patients represents 29 tissue cores from histological excisions, 23 microdissected biopsies and 3 whole-section biopsies which were compared to 45 microdissected cytological smears and 17 microdissected cell blocks (Supplementary Table S1).
Diagnoses of the biopsy (CIN III) and the subsequent cone biopsy were compared.
Tissue microarrays served as "biopsy procedure" and were compared with 454 whole tissue sections obtained from the same paraffin blocks used for the generation of tissue microarrays.
