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Liver biopsy sections were collected as previously described [ 24] and histological diagnosis of NAFLD according to Brunt et al [ 44] was used to select patients with histologically normal livers (grade 0, < 5% of steatotic hepatocytes; n=30) or with simple steatosis (NAS, grades 1 and 2, 5 to 66% of steatotic hepatocytes; n=26).
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To rule out the confounding effects of an occult leukemia, glioma or pheochromocytoma, a complete blood count, lymph node sampling, spleen biopsy, sternum bone marrow, adrenal gland biopsy and whole-brain sections were collected and examined histologically.
8-μm-thick unfixed cryostat muscle sections were collected from diagnostic muscle biopsies.
Some of the biopsies were processed into paraffin blocks, and ten 5μm sections were collected from each block and mounted on 3-aminopropyltriethoxysilane (TESPA; Sigma -coated Sigma -coated
To evaluate SOX4 protein expression, paraffin-embedded tissue sections were collected from 95 patients who had undergone diagnostic biopsy or definitive surgery for OSCC at Chonnam National University Hwasun Hospital (Jeonnam, Korea) between May 2004 and June 2013.
Four µm thick tissue sections were collected at a regular distance of 40 µm between biopsies.
Forty-micron sections were collected.
Sections were collected in PBS.
Sections were collected in 0.1 M PBS.
Sections were collected on nickel grids.
The sections were collected and carefully weighed.
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