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Portions of the biopsy samples were prepared for molecular analyses.
As learning cases, 18 biopsy samples were prepared (Table 1), including nine samples from Group R patients and nine samples from Group S patients.
Reconstituted biopsy samples were prepared in sterile 5-ml tubes (Nunc; Roskilde, Denmark) by adding 10 μl serial P aeruginosa dilutions (see above) to 50 mg uninfected cadaveric skin, at final concentrations of 10-10 CFU/g tissue.
Similar(57)
Formalin-fixed and paraffin-embedded (FFPE) tumour samples were prepared from primary surgical or biopsy specimens in lung.
The extracts from tissue samples were prepared in a similar manner, with the biopsy samples being snap frozen in liquid nitrogen and stored at −80°C until required.
No biopsies were available for these patients because they presented no deterioration of graft function (certain cDNA samples were prepared by TcLand Expression S.A., Nantes, France).
Samples were prepared in quintuplet.
Samples were prepared in triplicates.
Samples were prepared in triplicate.
Samples were prepared in duplicate.
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