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All collected biopsy samples were immediately placed in five ml of RNA-later solution (Qiagen, Valencia, CA) to preserve the RNA.
All biopsy samples were immediately weighed and snap frozen in liquid nitrogen-cooled isopentane, and stored at -80C for subsequent analyses.
Tissue samples obtained by surgery were snap-frozen in liquid nitrogen right after removal and biopsy samples were immediately collected in RNAlater™ (Ambion, Austin, TX).
The biopsy samples were immediately placed in RNA isolation buffer (Macherey-Nagel GmbH & Co. KG, Düren, Germany), snap-frozen in liquid nitrogen, and stored at −80°C to await further analysis.
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A series of fresh biopsy samples was immediately frozen in liquid nitrogen and then stored at −70°C.
After biopsies or surgical resections, tissue samples were immediately sent to the laboratory for Raman measurements.
The tissue samples were immediately frozen after biopsy for subsequent RNA and protein extractions, using Trizol (Invitrogen, Grand Island, NY) and Nuclear Factor kappa b (NF-κb) Lysis Buffer (Active Motif, Carlsbad, CA) respectively.
For nucleic acid analysis, sub-samples from each biopsy sample were fractured on dry ice and 50 100 mg samples were immediately homogenized with 1 ml Trizol (Invitrogen™).
The samples were immediately stored at −80 °C.
All samples were immediately preserved with HgCl2.
Samples were immediately stored at −80°C.
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