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When discussing histological criteria, it was specified that biopsy samples should be taken from the edge of an erosion where representative histology would most likely be present.
When resistance is suspected, biopsy samples should be analyzed by using molecular assays that enable rapid identification of mutations associated with drug resistance directly from paraffin-embedded biopsy specimens.
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For instance, in a study of chronic hepatitis C in which liver samples are obtained percutaneously, the histologically normal samples should be obtained through percutaneous liver biopsy.
It has also been suggested that PCR of CSF samples should be able to replace brain biopsies for some infection tests [14].
In patients with clinical and/or histopathological findings suggestive for DH, a biopsy of perilesional skin for DIF should be performed and serum samples should be collected to test anti-tTG antibodies (together with total IgA dosing).
Therefore, ideally, normal cervical samples instead of cervicitis samples should be used, however, these samples are difficult to obtain as biopsies are only performed when there is suspicion of lesion.
As with any transbronchial biopsy, the actual sampling should be done with fluoroscopic visualization to observe the position of the biopsy tools.
Taken together, it may well be hypothesised that tumour tissue is not the relevant starting material and the liquid biopsy represented by a blood sample should be the material of choice.
If the nodule is peripheral and smaller than 2 cm, the sample should be taken via a transparietal fine needle biopsy (option).
More than one tissue sample should be taken and it is also advisable to assess the characteristics of these samples at biopsy.
The diagnosis of ductal carcinoma in situ, lobular carcinoma in situ and atypical hyperplasia from a needle biopsy sample should always be reconfirmed using a sample obtained by surgical excision (recommendation, level of evidence: B1).
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