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We compared percentage present calls, absent calls, reproducibility, and concordance in needle biopsy samples processed by standard microarray protocol (cDNA non-amplification method) and our modified protocol (cDNA amplification method).
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Formalin-fixed and paraffin-embedded biopsy samples were processed by the avidin biotinylated peroxidase complex method (Chem Mate Detection kit; Dako Cytomation, Carpinteria, CA, USA).
Renal biopsy samples were processed by standard techniques for immunochemistry; clinical diagnosis was made on the basis of the immunochemical straining.
Endoscopic biopsy samples were processed into paraffin blocks, and H&E-stained slides were prepared, which were reviewed by independent expert histopathologists (MS and AN).
Renal biopsy samples were processed using the standard technique for transmission electron microscopy.
Biopsy samples were processed in a similar manner using a Qiagen DNA mini kit.
Biopsy samples were processed for routine histology and for immunostaining with the lysosomal marker LAMP2 and the autophagosomal marker LC3.
Prechemotherapy tissue samples obtained by tru-cut biopsy were processed by formaldehyde fixation and paraffin embedding.
Samples were processed by the Sydney University Proteome Research Unit.
These samples were processed by IHC.
Samples were processed by the same investigator.
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