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No bone marrow biopsy sample was available from patient with SLIT2 and NRP1 mutations.
To test this hypothesis, we assessed the presence of HER2 amplification by FISH, normalised for CEP 17, on CTCs from 75 women with breast cancer for whom the HER2 status of the primary cancer and a metastatic biopsy sample was available (patient characteristics available in Supplementary Table S1).
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§No cutaneous biopsy samples were available.
Of 4 patients for whom swab and skin biopsy samples were available, 3 had positive results.
Patients with unresectable primary lesion were eligible only when sufficient biopsy samples were available for chemosensitivity testing.
The samples were originally collected for a clinical study of EGFR mutations, for which the statuses of the biopsy samples were available.
No skeletal muscle and cardiac biopsy samples were available to perform additional studies to check for perturbed splicing of the DES mRNA transcript.
For some patients (1, 2, 3, 4, 5 and 7), repeat muscle biopsy samples were available, whereas for others (6 and 8) tissue from only a single biopsy was taken.
All the pre-operative biopsy samples were available but post operative surgical samples were available in only 75 patients due to the operations taking place in other hospitals, poor specimen quality or total tumor regression after CRT.
No infant sample was available.
However, only 11 biopsy tissue samples were available.
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